# Question 5.7: A fifth spectrophotometric method for the quantitative deter...

A fifth spectrophotometric method for the quantitative determination of the concentration of $Pb^{2+}$ in blood uses a multiple-point standard addition based on equation 5.6. The original blood sample has a volume of 1.00 mL, and the standard used for spiking the sample has a concentration of 1560 ppb $Pb^{2+}$. All samples were diluted to 5.00 mL before measuring the signal. A calibration curve of $S_{spike}$ versus $V_s$ is described by

$S_{spike} = 0.266 + 312 mL^{–1} × V_s$

Determine the concentration of $Pb^{2+}$ in the original sample of blood.

$S_{spike}=k\left\lgroup C_A\frac{V_0}{V_\mathrm{f}}+C_S\frac{V_s}{V_\mathrm{f}} \right\rgroup$     (5.6)

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