# Question 5.7: A fifth spectrophotometric method for the quantitative deter...

A fifth spectrophotometric method for the quantitative determination of the concentration of Pb^{2+} in blood uses a multiple-point standard addition based on equation 5.6. The original blood sample has a volume of 1.00 mL, and the standard used for spiking the sample has a concentration of 1560 ppb Pb^{2+}. All samples were diluted to 5.00 mL before measuring the signal. A calibration curve of S_{spike} versus V_s is described by

S_{spike} = 0.266 + 312 mL^{–1} × V_s

Determine the concentration of Pb^{2+} in the original sample of blood.

S_{spike}=k\left\lgroup C_A\frac{V_0}{V_\mathrm{f}}+C_S\frac{V_s}{V_\mathrm{f}} \right\rgroup (5.6)

**"Step-by-Step Explanation"**refers to a detailed and sequential breakdown of the solution or reasoning behind the answer. This comprehensive explanation walks through each step of the answer, offering you clarity and understanding.

Our explanations are based on the best information we have, but they may not always be right or fit every situation.

**blue check mark**means that this solution has been answered and checked by an expert. This guarantees that the final answer is accurate.

Learn more on how we answer questions.

## Related Answered Questions

Question: 5.13

## Verified Answer:

Once again, as you work through this example, reme...

Question: 5.12

## Verified Answer:

The equation for a normal calibration curve using ...

Question: 5.11

## Verified Answer:

Again, as you work through this example, remember ...

Question: 5.10

## Verified Answer:

Equations 5.13 and 5.14 are written in terms of th...

Question: 5.9

## Verified Answer:

To determine the concentration of Pb^{2+}[/...

Question: 5.8

## Verified Answer:

Equation 5.11 allows us to calculate the value of ...

Question: 5.6

## Verified Answer:

We begin by rewriting equation 5.6 as
S_{sp...

Question: 5.5

## Verified Answer:

The concentration of Pb²^+ in the o...

Question: 5.4

## Verified Answer:

The concentration of Pb^{2+} in the...

Question: 5.3

## Verified Answer:

To determine the concentration of Pb^{2+}[/...