# Question 5.4: A third spectrophotometric method for the quantitative deter...

A third spectrophotometric method for the quantitative determination of the concentration of Pb^{2+} in blood yields an S_{samp} of 0.193 for a 1.00-mL sample of blood that has been diluted to 5.00 mL. A second 1.00-mL sample is spiked with 1.00 μL of a 1560-ppb Pb^{2+} standard and diluted to 5.00 mL, yielding an S_{spike} of 0.419. Determine the concentration of Pb^{2+} in the original sample of blood.

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