## Chapter 17

## Q. 17.5

**Correcting Absorbance for the Effect of Dilution**

The absorbance measured after adding 125 μL (= 0.125 mL) of ferric nitrilotriacetate to 2.000 mL of apotransferrin was 0.260. Calculate the corrected absorbance that should be plotted in Figure 17-10.

## Step-by-Step

## Verified Solution

The total volume was 2.000 + 0.125 = 2.125 mL. If the volume had been 2.000 mL, the absorbance would have been greater than 0.260 by a factor of 2.125/2.000.

Corrected absorbance = (\frac{2.125 mL}{2.000 mL})(0.260) = 0.276

The absorbance plotted in Figure 17-10 is 0.276.

* Test Yourself* In a different titration, the absorbance after adding 75 μL of ferric nitrilotriacetate to 1.500 mL of apotransferrin was 0.222. Find the corrected absorbance.

(

*0.233)*

**Answer:**