Question 21.6: Classify the enzyme used in each of the following reactions....
Classify the enzyme used in each of the following reactions.
Analysis
Concentrate on the functional groups that change in the reaction, and use Table 21.3 to classify the enzyme.
Table 21.3 Classification of Enzymes | |
Enzyme Class or Subclass | Reaction Catalyzed |
Oxidoreductases | Oxidation–reduction |
• Oxidases | • Oxidation |
• Reductases | • Reduction |
• Dehydrogenases | • Addition or removal of 2 H’s |
Transferases | Transfer of a group |
• Transaminases | • Transfer of an \mathrm{NH}_2 group |
• Kinases | • Transfer of a phosphate |
Hydrolases | Hydrolysis |
• Lipases | • Hydrolysis of lipid esters |
• Proteases | • Hydrolysis of amide bonds in proteins |
• Nucleases | • Hydrolysis of nucleic acids |
Isomerases | Isomerization |
Lyases | Addition to a double bond or elimination to give a double bond |
• Dehydrases | • Removal of \mathrm{H}_2 \mathrm{O} |
• Decarboxylases | • Removal of \mathrm{CO}_2 |
• Synthases | • Addition of a small molecule to a double bond |
Ligases | Bond formation accompanied by ATP hydrolysis |
• Carboxylases | • Bond formation between a substrate and \mathrm{CO}_2 |

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a. The reaction involves the loss of a molecule (\mathrm{CO}_2 ) from a double bond, so the enzyme is a lyase. Because \mathrm{CO}_2 is removed, the lyase is subclassified as a decarboxylase.
b. The reaction involves hydrolysis—that is,cleavage of a bond with water—so the enzyme is a hydrolase. Because hydrolysis cleaves the amide bond in a dipeptide, the hydrolase is subclassified as a protease.
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