Question 21.6: Classify the enzyme used in each of the following reactions....

Classify the enzyme used in each of the following reactions.

Analysis

Concentrate on the functional groups that change in the reaction, and use Table 21.3 to classify the enzyme.

Table 21.3 Classification of Enzymes
Enzyme Class or Subclass Reaction Catalyzed
Oxidoreductases Oxidation–reduction
• Oxidases • Oxidation
• Reductases • Reduction
• Dehydrogenases • Addition or removal of 2 H’s
Transferases Transfer of a group
• Transaminases • Transfer of an \mathrm{NH}_2 group
• Kinases • Transfer of a phosphate
Hydrolases Hydrolysis
• Lipases • Hydrolysis of lipid esters
• Proteases • Hydrolysis of amide bonds in proteins
• Nucleases • Hydrolysis of nucleic acids
Isomerases Isomerization
Lyases Addition to a double bond or elimination to give a double bond
• Dehydrases • Removal of \mathrm{H}_2 \mathrm{O}
• Decarboxylases • Removal of \mathrm{CO}_2
• Synthases • Addition of a small molecule to a double bond
Ligases Bond formation accompanied by ATP hydrolysis
• Carboxylases • Bond formation between a substrate and \mathrm{CO}_2
21.6
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a. The reaction involves the loss of a molecule (\mathrm{CO}_2 ) from a double bond, so the enzyme is a lyase. Because \mathrm{CO}_2 is removed, the lyase is subclassified as a decarboxylase.

b. The reaction involves hydrolysis—that is,cleavage of a bond with water—so the enzyme is a hydrolase. Because hydrolysis cleaves the amide bond in a dipeptide, the hydrolase is subclassified as a protease.

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