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Question 21.4: In an original DNA strand, a segment has the base sequence 5...

In an original DNA strand, a segment has the base sequence 5′ A G T C C G 3′ .

a.  What is the sequence of nucleotides in the daughter DNA strand that is complementary to this segment?

b.  Why would the complementary sequence in the daughter DNA strand be synthesized as Okazaki fragments that require a DNA ligase?

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a.  Only one possible nucleotide can pair w ith each base in the original segment. Thymine will pair only with adenine, whereas cytosine pairs only w ith guanine to give the complementary base sequence: 3′ T C A G G C 5 ‘.
b.  Because DNA polymerase only adds nucleotides in the 5′ to 3′ direction, DNA on the lagging strand is synthesized as short Okazaki fragments, which are joined by DNA ligase.

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