Question 21.7: Protein Characteristics You have an aqueous solution of seru...
Protein Characteristics
You have an aqueous solution of serum albumin at pH 7, and currently the proteins are soluble. If you had 1M HCl and 1M NaOH at your disposal, what could you do to precipitate the albumin out of solution?
Strategy
Determine the pI of the protein and change the pH of the solution so that the protein reaches its isoelectric point.
The solution is at pH 7, and the pI of serum albumin is 4.9. Therefore, you would add HCl to the solution until the pH is 4.9, or until you notice the precipitate forming.
■ Quick Check 21.6
You have an aqueous solution of hemoglobin at pH 5.5, and currently the proteins are soluble. If you had 1M HCl and 1M NaOH at your disposal, what could you do to precipitate the hemoglobin out of solution?
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