Question 8.11: What is the pH of a solution if you mix 100. mL of 0.20 M HE...
What is the pH of a solution if you mix 100. mL of 0.20 M HEPES in the acid form with 200. mL of 0.20 M HEPES in the basic form?
Strategy
To use the Henderson-Hasselbalch equation, you need the ratio of the conjugate base to weak acid forms of the buffer. Since the HEPES solutions have equal concentrations, the ratio of the volumes will give you the ratio of the moles used. Divide the volume of the conjugate base form by the volume of the weak acid form. Take the log of the ratio and add it to the pK_a for HEPES
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