What is the sequence of a newly synthesized DNA segment if the template strand has the sequence
3’–TGCACC–5′?
Analysis
The newly synthesized strand runs in the opposite direction, from the 5′ end to the 3′ end in this example. Use base pairing to determine the corresponding sequence on the new strand: A pairs with T and C pairs with G.
Replication proceeds along both strands of unwound DNA. The leading strand grows continuously, while the lagging strand must be synthesized in fragments that are joined together by a DNA ligase enzyme.
