Question 5.9: A seventh spectrophotometric method for the quantitative det...

A seventh spectrophotometric method for the quantitative determination of Pb^{2+} levels in blood gives a linear internal standards calibration curve for which

\left\lgroup\frac{S_A}{S_{IS}} \right\rgroup_{stand} =(2.11  ppb^{-1})\times C_A-0.006

What is the concentration (in ppb) of Pb^{2+} in a sample of blood if (S_A/S_{IS})_{samp} is 2.80?

 

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To determine the concentration of Pb^{2+} in the sample of blood, we replace (S_A/S_{IS})_{stand} in the calibration equation with (S_A/S_{IS})_{samp} and solve for C_A

C_A=\frac{(S_A/S_{IS})_{samp}+0.006}{2.11  ppb^{-1}} =\frac{2.80+0.006}{2.11  ppb^{-1}} =1.33  ppb

The concentration of Pb^{2+} in the sample of blood is 1.33 ppb.

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